How to use differents region to assign content

Hi to all, i´m new in drupal

I´m starting to develop a site, in which need to display differents  contents in three regions, to do this I declare three diferents regions, left, rigth ant content.

I  use content use to display a unique block that only displaying in the home, and on the second of the site I need to display  a two column design left for menu and right for content.

The problem is that I can´t separate my content from the region-content, and this provocate that my two column second level don´t work.

Exist any possiblity to do this, or in another words is it possible to do with  only one theme.

Thanks in advance

Drupal version: